JONBENET Ramsey’s brother has broken his decade-long silence to insist crucial DNA evidence proves he had nothing to do with his little sister’s murder.

Burke Ramsey, 39, believes investigators are sitting on the clue that could finally identify the killer nearly 30 years after the six-year-old beauty queen was found ᴅᴇᴀᴅ.

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Burke, who was just nine at the time, has spent decades facing suspicion over the unsolved killing, despite never being charged.

Now, ahead of a new Diane Sawyer investigation airing on Friday, he has urged detectives to retest DNA recovered from his sister’s body.

“The DNA says we didn’t do it. I know none of my family did it. I didn’t do it,” Burke told the Daily Mail in an interview.

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“It’s like, stop. There was this energy looking at the family, but the DNA is sitting right there.”

He pointed to unidentified male DNA found on JonBenet’s underwear and beneath her fingernails, which he believes holds the answer to the decades-old mystery.

“We need to find that match. It’s the best evidence we have,” he said.

“It was underneath her fingernails, and it didn’t match any of us.”

Burke believes the unknown DNA could belong to his sister’s killer and wants the samples examined again using modern forensic technology.

“It’s probably the person who did it, and it’s the best chance we have to solve this. And I want it solved,” he said.

The 39-year-old acknowledged that he struggles to follow the forensic details because revisiting his sister’s death remains deeply painful.

“I don’t know much about the scientific nitty-gritty, and it’s hard to learn the details of the case because it is emotionally taxing,” he explained.

“But they need to send those samples to different labs again and keep testing.”

“Find the match. That will go a long way to bringing justice. It’s been 30 years and enough is enough,” he added.

The unidentified genetic material has been a major focus of the investigation for years, although its source and significance remain disputed.

In July 2008, then-Boulder County District Attorney Mary Lacy announced that DNA testing had cleared Burke and his parents, John and Patsy Ramsey, of suspicion.

The genetic material did not match any of the family members, leading Lacy to suggest an unidentified man was responsible.

However, the idenтιтy of the person who left the DNA has never been established, and the case remains unsolved.

Burke also spoke about the emotional toll of repeatedly being dragged back into the case.

“It’s the most tragic, personal, awful thing to happen in my life, that anybody could imagine happening to them,” he told the Daily Mail.

He said renewed attention every decade brings back painful memories, but he remains determined to find out who killed his sister.

During that interview, Burke addressed the public suspicion surrounding his family and recalled the devastating morning he learned JonBenet was ᴅᴇᴀᴅ.

“I know people think I did it, that my parents did it,” he said at the time.

“I know that we were suspects.”

Later in 2016, Burke sued CBS for $750 million after a documentary suggested he was responsible for his sister’s death.

The defamation case was settled in 2019.

Burke has consistently denied any involvement in the killing.

He has also offered to cooperate with investigators as they continue pursuing new leads.

JonBenet death became one of America’s most infamous unsolved murder cases after her mother discovered a ransom note inside the family’s home on the morning of December 26, 1996.

The handwritten note demanded $118,000, the same amount John Ramsey had recently received as a work bonus.

Hours later, John discovered his daughter’s body in the basement.

She had suffered a severe head injury and was strangled with a cord тιԍнтened around her neck.

Duct tape had also been placed over her mouth.

The investigation drew intense scrutiny, with detectives examining the family’s movements and possible intruders.

JonBenét’s mother died of ovarian cancer in 2006, without learning who killed her daughter.

No one has ever been charged with the murder.

Burke’s comments come just a day before ABC airs a new two-hour investigation examining the mystery surrounding the unidentified DNA.

JonBenet Ramsey: Race Against Time, Who Is ‘Unknown Male 1’?, will be presented by Diane Sawyer and ABC News chief investigative correspondent Aaron Katersky.